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Data from: DNA damage induced during mitosis undergoes DNA repair synthesis

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Data from: DNA damage induced during mitosis undergoes DNA repair synthesis

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1 digital object.

Cite This Work

Gomez Godinez, Veronica; Kabbara, Sami; Sherman, Adria; Wu, Tao; Cohen, Shirli; Kong, Xiangduo; Yokomori, Kyoko; Berns, M.W. (2020). Data from: DNA damage induced during mitosis undergoes DNA repair synthesis. UC San Diego Library Digital Collections. https://doi.org/10.6075/J08W3BQK

Description

Abstract: Understanding the mitotic DNA damage response (DDR) is critical to our comprehension of cancer, premature aging and developmental disorders which are marked by DNA repair deficiencies. In this study we use a micro-focused laser to induce DNA damage in selected mitotic chromosomes to study the subsequent repair response. Our findings demonstrate that (1) mitotic cells are capable of DNA repair as evidenced by DNA synthesis at damage sites, (2) Repair is attenuated when DNA-PKcs and ATM are simultaneously compromised, (3) Laser damage may permit the observation of previously undetected DDR proteins when damage is elicited by other methods in mitosis, and (4) Twenty five percent of mitotic DNA-damaged cells undergo a subsequent mitosis. Together these findings suggest that mitotic DDR is more complex than previously thought and may involve factors from multiple repair pathways that are better understood in interphase.

Creation Date
  • 2010 to 2019
Date Issued
  • 2020
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Technical Details

The files are best viewed in the open source, image processing package, FIJI, which is freely available on https://fiji.sc/.

Funding

This work is supported in part by grants AFOSR Grant # FA9550-08-1-0384 and a gift from the Beckman Laser Institute Foundation (M.W.B.) and Minority Health and Health Disparities International Research Training (MHIRT) Program, NIH Grant MD-01485. This work was supported by the National Science Foundation [grant MCB-1615701 to K.Y.].

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Language
  • English
Identifier

Identifier: M.W Berns: https://orcid.org/0000-0002-9640-2885

Identifier: Shirli Cohen: https://orcid.org/0000-0002-0253-786X

Identifier: Veronica Gomez Godinez: https://orcid.org/0000-0002-7209-4718

Related Resource

    Primary associated publication

    • Gomez Godinez V, Kabbara S, Sherman A, Wu T, Cohen S, Kong X, et al. (2020) DNA damage induced during mitosis undergoes DNA repair synthesis. PLoS ONE 15(4): e0227849. https://doi.org/10.1371/journal.pone.0227849

    • Veronica Gomez Godinez, Sami Kabbara, Adria Sherman, Tao Wu, Shirli Cohen, Xiangduo Kong, Jose Luis Maravillas-Montero, Zhixia Shi, Daryl Preece, Kyoko Yokomori, Michael W. Berns (2020). DNA damage induced during mitosis undergoes DNA repair synthesis. bioRxiv. https://doi.org/10.1101/2020.01.03.893784

Data from: DNA damage induced during mitosis undergoes DNA repair synthesis
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