staging
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Library Digital Collections

Microscopy product ID: 1

Component

Segmentation

Description

Segmentation of dendritic tree from medium spiny neuron from the nucleus accumbens of the mouse using Neurolucida. Portions of the dendrite in which the spines were too dim to trace are indicated byt the gray *.

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Full resolution data file

File Size
  • 118 KB
File Format
  • ASCII
Description

Output of Neurolucida tracing program in ascii format (acc1.spines3c.asc).

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Reconstruction

Description

Medium spiny neuron from the mouse nucleus accumbens injected with Lucifer Yellow and Alexa 568 and reconstructed from a 3D opcial section series. The Alexa568 channel is shown here. The original data was acquired as a tiled mosaic that was stitched together in X-Y.

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Full resolution data file

File Size
  • 289 MB
File Format
  • GZIP
Description

Multi-image tiff file (~570 Mb) containing the optical section series from the tiled mosaic. Note that the name of the file (Proj_EricsMontage.tif) is not the same as the microscopy product basename (acc1).

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Animation

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Description

rotation loop of maximum intensity projection of spiny neuron in nucleus accumbens. Some dendrites are incomplete due to the thickness of the section.

Full metadata

CCDB XML

File Size
  • 13.8 KB
File Format
  • XML
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Product description (pdf)

File Size
  • 104 KB
File Format
  • PDF
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Collection
Cite This Work

Martone, Maryann; Bushong, Eric A. (2017). Microscopy product ID: 1. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0VM4C1X

Creation Date
  • Microscopy product: 2001-12-13. Experiment: 2001-12-13. Project: 2001-09-01.
Date Issued
  • 2017
Research Team Head
Researcher
Technical Details

Male. Strain: C57BL/6

Product type: Optical section series and mosaic. Microscopy type: multiphoton. Instrument: BioRad 1024 MRC Confocal

Funding

NIH

Series
Scientific Name
Anatomy
Topic

Formats

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Language
  • No linguistic content; Not applicable
Related Resource
Copyright

Public domain (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work may be used without prior permission.

Digital Object Made Available By

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

Last Modified

2023-05-22